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| Solution Information | help | |
| Enzyme: | ATP-dependent molecular chaperone HSP82 | |
| inhibitor: | BDBM34677 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | 1) Grow reporter strain in SD-ADE media overnight @ 37 degrees C. 2) Dilute cells to OD = 0.04 in SD-ADE media. 3) Add 20 ul of culture to each well of 384 white plate. 4) Pin 100 nL compounds into plates, using 5uM Radicicol as positive control. 5) Add 20 ul of 20uM DOC (steroid) in SD-ADE media to plates. 6) Incubate at 30 C for 75 minutes, with agitation. 7) Add 40 ul Gal-Screen reagent. 8) Incubate at 30C for 25 minutes, then read luminescence. | |
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